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Fig. 4. Hint1 is associated with the LEF-1–ß-catenin transcription complex. (A) HEK293 cells were transiently transfected with FLAG-tagged ß-catenin and myc₆-tagged Hint1 alone (lanes 1 and 2) or in combination (lane 3). After lysis immunoprecipitation was performed with anti-FLAG M2 and association of Hint1-myc₆ was analysed by western blotting with anti-myc (9E10) antibody. (B) Hint1-myc₆ associates with LEF-1–HA in HEK293 cell lysates as shown by co-immunoprecipitation with anti-HA (12CA5) and western blotting with anti-myc (9E10) antibody. **Heavy and *light chain of the precipitating antibody.

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