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Fig. 5. Hint1 and Pontin co-localize in the nucleus and are concentrated in speckled structures. Immunofluorescence images of H184A1 cells double-stained with mouse monoclonal anti-Pontin (5G3-11) (Aa, Ba') and guinea pig anti-Hint1 ({alpha}-A15DC) (Ab, Bb') antibodies taken with a fluorescence (A) and a confocal (B) microscope. (Ac) DAPI staining; (Bc') merge of images Ba' and Bb'. Arrows mark co-localization of Pontin and Hint1 in speckled structures. Bar, 10 mm.





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