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Fig. 1. Expression and distribution of IP₃ receptor isoforms in skeletal myotubes. (A) Representative western blots of six different experiments showing all three types of IP₃Rs (IP₃R1, IP₃R2 and IP₃R3) expressed in myotubes with a molecular mass of $~$ 260 kDa. No crossreactivity was seen. To correct for loading the blots were probed with an antibody that recognizes actin. (B) Upper panel, type-1 IP₃R labeling is distributed in a dotted pattern in the nuclear envelope region. Inset, labelling of a differentiated myotube; striations correspond to limited regions of sarcoplasmic reticulum. Center panel, labeling of type-2 IP₃R is distributed throughout the reticulum and absent from nuclei. Lower panel, labeling of type-3 IP₃R is concentrated in nuclei, mainly in condensed structures and also occurs faintly in the cytoplasm. Bars, 5 µm (top), 25 µm (center, bottom).

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