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Fig. 3. Characterization of an isolated nuclear fraction from skeletal myotubes. (A) Fluorescence images of isolated myonuclei from a primary culture immunolabeled with LAP2 showing the integrity of the nuclei after the purification procedure. (B) Representative western blots of three different experiments showing minimal contamination of the nuclear fraction with the sarcoplasmic reticulum markers calsequestrin (top), and triadin (middle), but enriched with the nuclear marker LAP2 (bottom). (C) Transmission electron micrograph of a purified nucleus, showing the conserved nuclear structure. (D) High magnification electron micrograph showing the continuity and integrity of the nuclear membrane. Bar, 20 µm (A); 2 µm (C); 0.5 µm (D).





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