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Fig. 6. IP₃ induced nucleoplasmic Ca²⁺ increases in isolated myonuclei. The time course of relative fluorescence change (ratio between the fluorescent difference, stimulated minus basal and the basal value) as a function of time is shown. (A) Stimulation of isolated myonuclei with 10 µM IP₃ caused a quick and transient increase in calcium ( ${bullet}$ ). Such an increase was inhibited by 2-APB ( ${blacksquare}$ ) and XeB ( ${circ}$ ). The physiological solution (vehicle) alone did not induce any signal ( ${triangleup}$ ). (B) Series of fluo-3 fluorescent images of myonuclei recorded at the times indicated. Basal fluorescence is shown in the top, the following image was taken immediately after 10 µM IP₃ was added. (C) Isolated nuclei from 1B5 myotubes, stimulated with 10 µM IP₃ display a quick and transient increase in calcium ( ${bullet}$ ), that was inhibited by 2-APB ( ${square}$ ) and XeB ( ${blacksquare}$ ). The vehicle IP₃ alone did not induce a signal ( ${circ}$ ). (D) Series of fluo-3 fluorescent images in 1B5 nuclei collected at the times indicated. Basal fluorescence is shown at the top, the images that follow were taken after the stimulus with 10 µM IP₃. (E) Fluorescence in a nucleus suspension using dyes reported to specifically locate to either the nuclear envelope (mag-fluo-4) or the nucleoplasm (fluo-4 dextran). The suspension was stimulated with 10 µM IP₃ as indicated by the bar, traces shown are representative of 20 different measures from four different preparations. Nuclear diameter is approximately 10 µm.

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