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Fig. 7. IP3 and calcium induced CREB phosphorylation in isolated myonuclei. Top panel, western-blots of CREB phosphorylation at Ser133 following additions of Ca2+ and IP3 solutions at the times indicated. Incubation conditions were similar to those described for Fig. 6C. To correct for loading, blots were probed with an antibody that recognizes the phosphorylated and non-phosphorylated forms of CREB. Bottom panel, fold induction (mean±s.e.m. of three different experiments) of CREB phosphorylation over control levels. Significant differences *P<0.05 and **P<0.01 in induction of phosphorylation were observed between control and IP3- and Ca2+-treated groups at the indicated time points.





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