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Fig. 4. Temperature dependence of GM1 distribution and the effect of Triton X-100 extraction. Jurkat T cells were fixed at the specified temperatures and, where indicated, extracted with 1% Triton X-100 on ice for 5 minutes. The cells were then stained with CT-B-rhodamine or CT-B-Alexa Fluor 594. Single equatorial confocal sections show fluorescence in the rhodamine channel. Arrowheads indicate plasma membrane regions where GM1 has been extracted. Scale bars: 5 µm.





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