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Fig. 7. Distribution of tyrosine phosphorylated proteins. A, TX-DRMs were prepared from Jurkat cells incubated and stimulated with 1 µg/ml OKT3 (+) or left unstimulated (–) at either 10 or 37°C. TX-DRMs from 107 cells were loaded on the gel. (B) TX-DRMs (DRM), Triton-soluble proteins (SP) and pellets (P) from cells incubated at 37°C were loaded on an equal cell basis. Molecular mass markers are indicated (kDa). Fluorographs shown are representative of two experiments.





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