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Fig. 1. Sf9 cells gain temporary Cry1C-tolerance during mitosis and early G1 phase. Log-phase Sf9 cells were incubated with 500 ng/ml Cry1C and photographed every 3 minutes. (A,B) Two microscopic fields with dividing cells (indicated by arrows) that were Cry1C-insensitive and progressed in their divisions. Inserts show the magnified regions (x2.3) to their right. After division and in the presence of Cry1C, daughter cells remained alive at least 300 minutes (A) or up to 22 hours (B). The other non-dividing cells gradually died within 180 minutes. A mean value of dividing cells in percent (1.58±0.95) was estimated by counting 10 different cell cultures.





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