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Fig. 2. G2-M-arrested Sf9 cells survived treatment with Cry1C. Normal Sf9 cells and G2-M-arrested Sf9 cells were exposed to increasing concentrations of Cry1C for 4 hours. Dead and viable cells were counted (three repeated experiments). Dose-dependent mortality curves (A) were used to estimate LC50 (B). The control curve, data from untreated cells exposed to a mixture of Cry1C (increasing concentrations) and nocodazole (10 µg/ml), indicates that nocodazole has no effect on Cry1C-activity.