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Fig. 4. Expression of hnRNP A1, A2, B1 and A3 in different cells. Western analysis of total protein extracts with rabbit polyclonal antibodies raised against the A1, A2, B1 and A3 peptides, using a mouse monoclonal GAPDH antibody as a loading control. The keratinocytes were isolated from normal foreskin. Colo16 and SCC25 are squamous cancer cell lines originated from tumours on foreleg and tongue, respectively. HeLa is a cervical cancer cell line and A549 was cloned from lung cancer. MCF10a is an immortalized non-tumorigenic breast cell line, and MCF7 and T-47D are two breast cancer cell lines. RPMEI is an immortalized prostate cell line and LNCAP was cloned from human prostate cancer.





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