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Fig. 6. shRNA suppression of hnRNPs A1, A2 and A3 in Colo16 cells. Colo16 cells were seeded in six-well plates (360,000 cells/well) and transfected 20 hours later. Control samples were treated with lipofectamine only. 72 hours after transfection, cells were collected and lysed. Total proteins were then separated on SDS-PAGE, transferred onto PVDF membrane and probed with antibodies against A1, A2 or A3 using GAPDH as a loading control.





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