JCS02452 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. (A) Western blot analysis to test anti-HOPS antibody. COS-1 cells transfected with empty vector CMV and different amounts of CMV-vector containing Hops cDNA. (B) Western blot analysis of HOPS-GFP fusion protein. Cos-1 cells transfected with empty vector (Control), with HOPS-GFP and GFP vector were detected with anti-HOPS and anti-GFP.

• Supplemental Figure 2 -

  Fig. S2. Imunolocalization of HOPS in H-35 stable clones overexpressing HOPS using anti-HOPS antibody. DAPI staining of the same slides. Merge of anti-HOPS and DAPI staining.