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Fig. 5. Effect of leptomycin B (LMB) on HOPS shuttling. (A) Comparison of NES sequence in HOPS and other proteins. (B) Immunohistological staining (anti-HOPS) of H-35 hepatoma cells left untreated (–LMB) or treated with LMB (+LMB) resulted in accumulation of HOPS in nucleus in treated cells. DAPI: DAPI staining of the same slides; merge: merged images of anti-HOPS and DAPI. (C) Quantification of HOPS staining in the nucleus and cytoplasm in the presence or absence of LMB. Data are representative of five separate experiments. Standard errors of the mean are indicated by error bars. Scale bars: 20 µm.





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