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Fig. 2. GFP:NtMAP65-1a signal recovery after photobleaching. (A) GFP:NtMAP65-1a signal in the hypocotyl of 7-day-old Arabidopsis seedlings. The white rectangle outlines the photobleached area. (B) Time series (top to bottom) of GFP:NtMAP65-1a signal recovery during a FRAP experiment within the area indicated by the rectangle in A. The numbers on the right-hand side indicate the time in seconds when each of the frames was collected with 0 corresponding to the image before the photobleaching onset and 4.766 just after the photobleaching. (C) The first FRAP measurement was taken just before the photobleaching and corresponds to point 0. The grey sector represents the duration of photobleaching, after which 14 images were collected and measured at approximately 4.14-second intervals. The fluorescence signal was measured in 24 cells and expressed as the mean percentage of the signal before photobleaching. The error bars indicate s.d. of the mean. Bar, 5 µm.





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