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Fig. 5. Atm^-/- Spo11^+/- testis sections show severe defects in spermatogenesis and substantial levels of apoptosis. (A) Atm^-/- Spo11^+/- testes show severe defects in spermatogenesis in spite of the prophase-I rescue. Paraffin-embedded testis sections from wild-type (a,b) and Atm^-/-Spo11^+/- (c-i) mice stained with hematoxylin and eosin. In wild-type sections, many round or elongated spermatids are found in the most lumenal part of the tubules. By contrast, in Atm^-/- Spo11^+/- testes, very few tubules contain elongated spermatids (d,e, arrows) and, even in positive tubules, only groups of two to 15 spermatids can be found. Examples of aberrant metaphases are frequently observed (f-i). (B) Metaphases frequently stain positive for TUNEL in Atm^-/- Spo11^+/- testes sections. Apoptotic cells were detected by TUNEL on paraffin-embedded testes sections. The DNA was stained with DAPI. (C) Aberrant metaphases of the first meiotic division can be observed in metaphase spreads from the Atm^-/- Spo11^+/- mutant. Metaphase spreads show metaphases of the first meiotic division containing autosomal univalents (marked with arrows), chromosomal fragments [asterisk (^*)], achiasmatic sex chromosomes and broken bivalents [hash (#)].

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