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Fig. 2. Chromatin loading assay of Ku80, Ku70 and MCM7 in HCT116 and Ku80^+/- cells. Chromatin-enriched extracts were prepared (as described in Materials and Methods) and subjected to electrophoresis on 5% stacking/8% separating gels and western blotting. The abundance of Ku80 and Ku70 in the chromatin-enriched fractions were assessed. Actin was used a loading control. (A) Immunoblotting with 1/100^th dilution of anti-Ku80 (C-20), 1/1000^th dilution of anti-Ku70 (C-19) or 1/1000^th dilution of anti-actin antibody is shown. (B) Quantification of the Ku80 and Ku70 signals after normalization for actin. Each error bar represents three experiments and 1 SD is indicated. Signals obtained for HCT116 were set at 100% and those of Ku80^+/- were expressed as a percentage of them. An asterisk (^*) represents statistically significant differences (P<0.05) in the expression of the indicated protein between HCT116 and Ku80^+/- cells.

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