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Fig. 3. Flow cytometric analysis of HCT116 and Ku80^+/- cells following synchronization to late G₁ and release into S-phase. Cells were synchronized to late G₁ by thymidine/mimosine double block (late G₁), and released into complete medium. They were harvested at 2 (S2), 4 (S4), 6 (S6) and 8 (S8) hours after release, and stained with propidium iodide to monitor their cell cycle distribution. An asynchronous culture of logarithmically growing cells (Log) was used for comparison. (A) Representative data from six experiments are shown. The top panel represents the wild-type (HCT116) cells, and the bottom panel is of the Ku80^+/- ones. (B) Quantification of the percentage of cells present in each phase of the cell cycle (G₁, S or G₂/M) during the synchrony (G₁) and release (S2-S8). Averages of six experiments and their standard deviations (in brackets) are shown. Cell cycle phases statistically different (P<0.05) between Ku80^+/- and HCT116 cells are shown in bold.

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