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Fig. 5. Maps of the lamin B2, ß-globin and c-myc origin loci, and location and characteristics of the LightCycler primers. (A) Maps of the lamin B2 (top left), ß-globin (top right) and c-myc (bottom left) regions. The location and names of the primers is indicated (arrows). For the lamin B2 locus, the exon (black boxes) numbers of the lamin B2 and ppv1 genes are indicated above. For the ß-globin locus, the name of each globin gene (black box) is indicated above. For the c-myc locus, the promoters (arrows) and exons (black boxes) of the c-myc gene are indicated. The distance between the origin-containing and origin-lacking amplicons are indicated in kb (double-headed arrows). (B) The melting curves of the new primers designed for the ß-globin and c-myc origins are shown. Two curves are shown with each primer pair, one containing (+DNA) and one lacking (-DNA) template DNA. None of the primer sets showed any nonspecific products. (C) Standard curves used for the quantification analysis by the LightCycler instrument for each of the four primer sets shown in B. The correlation coefficients (r²) and slopes are indicated. The similarity in the slopes indicates similar primer efficiencies between the different primer pairs.

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