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Fig. 7. Quantification of nascent strand abundance at three origins in Ku80+/- and wild-type cells. Nascent DNA from the 0.5-1 kb fraction prepared from asynchronous cultures of HCT116 and Ku80+/- cells as in Fig. 5. Origin activity of the lamin B2 (A), ß-globin (B) and c-myc (C) origins were measured by quantification of the nascent strand abundance at each origin. LB2, BG40.9 and Myc11 are located within the origins whereas LB2C1, BG72 and Myc1 are origin-lacking controls, located distant to the origins (see Fig. 4A for maps). Each error bar represents three experiments and 1 SD is indicated. An asterisk (*) represents a statistically significant difference (P<0.05) between HCT116 and Ku80+/- cells in the abundance of nascent DNA at the specified DNA regions.





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