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Fig. 8. Association of Ku70, Ku80 and Orc2 to the lamin B2, ß-globin and c-myc origins in HCT116 cells. Asynchronous cultures of HCT116 cells were treated with 1% formaldehyde and harvested. Chromatin fractions were immunoprecipitated with either anti-Ku80, -Ku70 or -Orc2 antibodies, or normal goat serum (NGS) as control. (A) Western blot analyses of the immunoprecipitates with the immunoblotting antibody indicated on the left and the immunoprecipitating one above the figure. (B) The abundance of DNA for three origins (LB2, BG40.9 and Myc11) along with their origin-lacking controls (LB2C1, BG72 and Myc1) in each of the precipitates was determined using realtime PCR, thus measuring the association of each protein with the examined DNA region. The average of three experiments and 1 SD is shown. An asterisk (*) represents statistically significant differences (P<0.05) in the association of the indicated protein between the origin-containing and origin-lacking regions.





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