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Fig. 1. Integrin expression profile and adhesion to LM609. (A) Parental CHO cells (CHO{alpha}V) and clonal CHO{alpha}Vß3 cells were subjected to (a) western blot analysis for {alpha}V and ß3 subunits (primary antibodies diluted 1:300), and (b) to FACS analysis for {alpha}Vß3, ß1, {alpha}Vß5 and {alpha}Vß6 integrin expression profile. Grey shadows, fluorescence background; black traces, integrin-specific fluorescence intensity. (B) CHO{alpha}V and CHO{alpha}Vß3 cells were allowed to adhere to immobilized LM609 monoclonal antibody or FBS for the time indicated in graphs (left panels, crystal violet staining), or for 30 minutes for morphological analysis (middle and right panels, phase contrast microscopy). Original magnification 10x. (C) Osteoclast cultures (including TRAcP-positive mature multinucleated cells and putative mononuclear precursors) were obtained from bone marrow as described in Materials and Methods, gently lifted in 0.02% EDTA-containing buffer and allowed to attach to immobilized LM609 or FBS for the time indicated in graph (left panel) or for 30 minutes for morphological analysis (middle and right panels, staining for TRAcP). Original magnification 20x.





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