|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. Co-repressors regulate Pit-1-dependent transcription. Luciferase reporter-gene transcription assays were performed using (A-C) GH4ZR7 pituitary cells or (D-F) non-pituitary HeLa cells. Cells were transfected with the indicated reporter vectors and expression vectors. The panels show the relative luciferase activity, corrected for total cellular protein in the lysates. Results are the means of measurements from at least three cultures and error bars denote the s.e.m. (B,D) Statistical analysis was performed with P<0.05 considered to be significant. The lower-case letters indicate sets of measurements that are statistically different. (D, insert) Western-blot analysis of HeLa cells transfected with the indicated expression vectors. (G) Lysates were prepared from cells transfected with the indicated expression vectors. Immunoprecipitations were performed using an anti-HA agarose conjugate and anti-GFP antibody was used for western blot analysis.
|
