Characteristics of subepithelial fibroblasts as a mechano-sensor in the intestine: cell-shape-dependent ATP release and P2Y1 signaling
J Cell Sci Furuya et al.
118: 3289
Movie 1
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Movie 1. A
Slight touch with a blunted thin glass rod induced intercellular Ca2+-waves
in subepithelial fibroblasts. Two images of indo-1 fluorescence (F405 and F480)
were superimposed onto a Nomarski image where F405 was represented by Red and
F480 by Green. So, cells in which Ca2+ increased turned red. Note;
cell contractions are also propagated concomitantly with Ca2+ wave
propagation.
Movie 2
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Movie 2.
Touching stimulation induces cellular contractions that propagate to
surrounding cells like a wave. In some cells, transient bleb formation in the
cell membrane was observed. Same images as shown in Fig 4.
Movie 3
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Movie 3.
Mechanical stimulation, ‘stretch’, induces Ca2+ responses and
following Ca2+ waves. Stretch stimulation (12 % for 3 s) was applied
to vertical direction in the figures. Ca2+ responses are shown by
merged RGB color images of F405 (as Red), F480 (as Green) and F405/F480 (as
Blue). Same images as shown in Fig 5.
