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Fig. 3. The effect of agonists and antagonists of ATP receptors on Ca²⁺ responses. Intracellular Ca²⁺ responses shown by indo-1 fluorescence ratios (F405/F480), were measured in subepithelial fibroblasts cultured for 3 days using a laser confocal microscope. Color traces show responses in each cell and black traces with filled circles indicate the average response. (A) Ca²⁺ responses to UTP, ATP, UDP and ADP. The nucleotide concentrations were 10 µM. ATP and ADP are equally potent to induce Ca²⁺ increase, UTP is less effective, and UDP is not effective at 10 µM. (B) In Ca²⁺-free solution, a similar Ca²⁺ response to ATP was initially observed, but later was suppressed. (C) 2MeSATP, a P2Y1 agonist, is more effective than ATP. The responses to each agonist at concentration 0.1 µM are compared. In the same cells, carbachol, a cholinergic agonist, is not effective even at 100 µM. (D) MRS2179 (30 µM), a P2Y1 antagonist, potently blocks the Ca²⁺ response to 2MeSATP (1 µM). The response is recovered after 10 minutes washout of MRS2179. Conversely, MRS2159 (100 µM), a P2X1 antagonist, is not effective on the Ca²⁺ response.

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