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Fig. 6. Subcellular localization of Grp during embryonic cell cycles. Grp localization during syncytial divisions. 1.5-2.5-hour-old wild-type embryos were fixed and stained with an antibody to Grp (
-Grp), wheat germ agglutinnin (WGA) to visualize nuclear envelopes and Hoechst33258 to visualize DNA. Grp is nuclear during interphase and disperses from the nucleus as the nuclear envelope breaks down in mitosis. Grp re-accumulates in the nucleus after the nuclear envelope reforms in the next interphase.