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Fig. 5. Analysis of flagellar dynamics in Cnk2p-HA and RNAi strains. (A) Flagellar lengths were measured during recovery from pH shock in the presence or absence of 10 µg/ml cycloheximide. 40 flagella were measured for each time-point for each of three independent experiments, error bars represent s.e.m. (B) Flagellar lengths during resorption of flagella in fla10-1:Cnk2p-HA and fla10-1:CNK2-RNAi strains after shift to restrictive temperature. 40 flagella were measured for each time-point for each of three independent experiments, error bars represent s.e.m. (C) Cnk2p-HA cells assembling flagella post deflagellation and disassembling flagella prior to mitosis, triple-stained for indirect immunofluorescence with anti-{alpha}-tubulin, anti-HA and anti-centrin. Bar, 5 µm.





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