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Fig. 5. UNC-43(K148E) and UNC-43(D238R) fail to rescue unc-43 mutants for GLR-1 trafficking. (A-G) GLR-1::GFP in neurons cell bodies and (H-N) SNB-1::GFP in neurites were examined in unc-43 mutants that also express either (A,H) no transgene, (B,I) a wild-type unc-43 cDNA, or unc-43 cDNA with the (C,J) K148E, (D,K) D238R, (E,L) H282K, (F,M) R283E or (G,N) T286D mutations. GLR-1::GFP is found in perinuclear puncta (arrowheads), and accumulates to high levels in these puncta in unc-43 mutants carrying no transgene (A), or expressing UNC-43(K148E) (B) or expressing UNC-43(D238R) (C). (O) Fluorescence quantification measuring the mean fluorescent intensity values for neuronal cell bodies expressing GLR-1::GFP and the indicated UNC-43 transgene. (P) SNB-1::GFP cluster number in neurites expressing the indicated UNC-43 construct. Significant differences in fluorescence intensity were observed ^*P<0.05, ^**P<0.01 and ^***P<0.001 when compared to levels in the `None' group (no transgenic kinase) by one-way ANOVA followed by Bonferoni comparisons. The bar indicates a Bonferoni comparison between R283E and WT groups. n=10-30 animals per genotype.

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