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Fig. 6. UNC-2 regulates UNC-43 abundance in neurites. (A,C) Cell bodies and (B,D) neurites expressing wild-type UNC-43::GFP in (A,B) wild-type animals, or (C,D) in unc-2(e55) animals. Mutations in unc-2 result in the failure of UNC-43 to accumulate throughout the unlocalized neurite pool (D). Fluorescence quantification measuring the mean fluorescent intensity values for (E) neuron cell bodies and (F) neurites expressing UNC-43::GFP in either wild-type (WT) or unc-2(e55) mutant animals. (G) The number of clusters (clusters defined as above a threshold of two standard deviation values of the mean outside of the clustered signal) per 10 µm length of neurite for the indicated genotypes. A.U., arbitrary units. Error bars indicate s.e.m. Highly significant differences (^***P<0.0001) in cluster number and neurite levels were observed in unc-2 mutants compared to levels in the WT control group using the Student's t-test. n=8 animals for each genotype. Bar, 5 µm.

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