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Fig. 10. Tat protein induces internalization of TNF-R2. After immunostaining of TNF-R2 with a TNF-R2-specific antibody/Zenon Alexa Fluor 647 conjugate, HeLa-TNF-R2 cells were washed and incubated with fluorescein-labelled Tat protein. Cells were analysed by multichannel confocal microscopy; the first images were taken immediately (2 minutes) after protein addition and further images were taken as indicated. Scale bar: 20 µm.