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Fig. 9. Antp-mediated internalization of TNF-R2 occurs without recruitment of TRAF2. HeLa-TNF-R2 cells were transfected by electroporation with a plasmid coding for a TRAF2-GFP fusion protein. After 24 hours TNF-R2 was stained using a TNF-R2-specific antibody/Zenon Alexa Fluor 647 conjugate. After washing, cells were either stimulated with TNF-R2-specific mutant TNF (300 ng/ml, 30 minutes at 37°C) or incubated with unlabelled Antp (20 µM, 30 minutes at 37°C) or left untreated: (A-C) receptor staining; (D-F) TRAF2-GFP; (G-I) superposition of both channels. Scale bar: 20 µm.





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