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Fig. 2. F-dexa–binding to the membrane of single AtT-20 cells quantified by FCS. Intensity fluctuations and the measured and calculated corresponding autocorrelation curves G({tau}) at the cell membrane position for representative F-dexa concentrations of (a) 25, (b) 80 and (c)120 nM. Data were fitted with the three component model, with the fixed diffusion times of {tau}1=0.05, {tau}2=3.6 and {tau}3=255 ms. The resulting fractions were f1=0.61, f2=0.26, and f3=0.13 for 25 nM, f1=0.55, f2=0.16, and f3=0.30 for 80 nM, and f1=0.23, f2=0.21, and f3=0.56 for 120 nM.





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