spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Nuclear extracts from MDCK-Snail cells contain specific NF{kappa}B-binding complexes. Nuclear extracts from MDCK-CMV and MDCK-Snail cells were analysed in band-shift assays showing the binding of nuclear proteins to the 32P-labeled NF{kappa}B wild-type probe, in the absence or presence of 500-fold molar excess of wild-type (lanes 3 and 8) or mutant cold oligonucleotides (lanes 4 and 9) or in the presence of anti-p65 (lanes 6 and 11) or control mouse IgG (lanes 5 and 10). Lane 1 shows a control in the absence of the nuclear extract. White arrowheads indicate the complexes detected (i and ii). A black arrowhead indicates the supershifted complex. The complete sequence of the NF{kappa}B probe is shown at the bottom of the figure. The specific nucleotides mutated in the NF{kappa}B oligonucleotide are underlined. The gel shown is representative of at least two independent experiments.





Right arrow Return to article