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Fig. 6. Ets-1 and Sp-1(HA) elements are responsible for the increase in MMP-9 proximal promoter activity in MDCK-Snail cells. (A) Top, schematic representation of the proximal 5' region -97 bp from the initiation transcription site, indicating the position of the potential regulatory control elements Ets-1, Sp-1 low affinity (LA) or high affinity (HA) and TATA-like (grey box). Middle and lower sequences: point mutations and putative Ets-1 and Sp-1(LA or HA) binding sites are indicated in each mutant construction. (B) The wild-type pMMP9-97 construct (construct V^wt) or mutants (construct V^{M 89/87} and V^{M 85/82}) fused to the luc reporter gene, were co-transfected with TK renilla into MDCK-Snail cells. Luciferase activity of each construction was determined and represented as fold stimulation of the activity over the -97 MMP-9 promoter (construct V^wt) as the mean±s.d. of three independent experiments.

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