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Fig. 1. Expression of murine cathepsin L (CTSL) in skin from the backs of ctsl^+/+, ctsl^-/- and Tg(K14-CTSL);ctsl^-/- mice. (A) Transgene construct. Functional elements are the human keratin 14 promoter, the rabbit ß-globin intron, the full-length mouse CTSL cDNA, and the human growth hormone poly(A)signal. (B-D) Immunohistochemistry for detection of CTSL (brown staining; bars 20 µm). (B) Wild type; (C) Tg(K14-CTSL);ctsl^-/-; and (D) ctsl^-/-. (E) RT-PCR (endpoint amplification; 50 cycles) for detection of CTSL and GAPDH mRNA in primary dermal fibroblasts and epidermal keratinocytes. Lanes 1 and 8: 100 bp ladder; lanes 2 and 5: ctsl^+/+; lanes 3 and 6: Tg(K14-CTSL);ctsl^-/-; lanes 4 and 7: ctsl^-/- (representative of three independent experiments). (F) Quantitative RT-PCR (real-time) for quantification of CTSL and GAPDH mRNA in liver, kidney and epidermis of wild type (ctsl^+/+) and Tg(K14-CTSL);ctsl^-/- mice (n=3). (F) CTSL proteolytic activity in epidermal lysates of wild type (ctsl^+/+) and Tg(K14-CTSL);ctsl^-/- mice (n=2).

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