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Fig. 5. CTSL-knockout keratinocytes are more responsive to EGF stimulation than wild-type cells. Proliferation of ctsl^+/+ (pale gray columns) and ctsl^-/- keratinocytes (dark gray columns) grown (A) in absence (no EGF) or (B) in presence (EGF) of exogenously added murine EGF (10 ng ml^-1). The last supplementation with EGF was performed simultaneously with the addition of [³H]thymidine. ^*P<0.05 compared with ctsl^+/+ keratinocytes. (C) Western blot for detection of EGF-receptor (EGFR) on keratinocytes and (D) quantitative RT-PCR (real-time) for quantification of EGF-receptor and GAPDH mRNA in absence of exogenous EGF.

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