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Fig. 1. Short intranuclear spindles are maintained throughout the cell cycle. S. neurona-infected cultures were double labeled with anti-tubulin (A,D) and anti-centrin (B,E) antibodies to detect microtubules and centrosomes, respectively. (C,F) The merged images of the red (tubulin), green (centrin) and blue (DAPI) channels. Centrosomes organize a complex network of spindle microtubules during mitosis (A-C). The `dots' of tubulin staining observed during interphase are flanked by two centrosomes (D-F, also see inset in F). (G-I) S. neurona-infected cultures were fixed in situ, embedded in Epon and ultrathin sections were cut for electron microscopy. (G) A section through a young schizont. The nucleus (N) is located at the center of the cell and plastid (P) and Golgi (G) are equally discernible. (H,I) Serial sections of the boxed region of G at higher magnification. A short basket of microtubules is evident within the nucleus (black arrowhead), which emanates from a centrosome on the cytoplasmatic site of the nuclear envelope (white arrowheads with black outline). Interestingly the end of this minute spindle seems to be in contact with condensed nuclear material (white arrows).
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