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Fig. 2. S. neurona forgoes nuclear division and cytokinesis for five cell cycles prior to the budding of 64 daughter cells. Cell cultures were infected with S. neurona merozoites and fixed and processed for immunofluorescence 24-72 hours after infection. Cells were incubated with a monoclonal antibody against 
-tubulin (red) and DAPI to stain DNA (blue). Developing schizonts present two mutually exclusive staining patterns: multiple spindles throughout the nucleus coinciding with DNA condensation or dots in proximity to the nuclear envelope (see Fig. 1). The number of these dots increased with the size of the schizont in a geometric progression (2-64, A-F). (G) Plot of the intensity of DNA staining of each nucleus (in arbitrary units, as shown for a parasite and host cell nucleus in inset) against the number of tubulin dots per nucleus (100 random fields were analyzed, individual measurements are shown as red dots, black lines and error bars indicate the mean and standard deviation, respectively). Progression from 32 to 64 (E,F) produces 64 daughter nuclei. Note that the dots often appeared as doublets (D).
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