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Fig. 6. Ablation of spindles perturbs plastid organization and faithful segregation, but does not prevent fission. (C-F) Infected cultures were allowed to develop for 48 hours and then treated with 2.5 µg/ml oryzalin for 24 hours and stained for plastids using an anti-ACP antibody. (A,B) Untreated controls. The plastid loses its organization and attachment to the nuclear envelope (D,F) but remains associated with centrosomes detected by centrin staining (E). (G,H) After 48 hours of drug treatment plastid fission is observed in some schizonts. (I-L) Labeling of identically treated cultures with an antibody to IMC3 shows that daughter cell budding still occurs (K), however when compared to controls (I,J) treated schizonts seem unable to segregate nuclei and produce anucleate daughter cells (L). (H) Contact of nuclei and plastids is equally lost.
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