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Fig. 6. GFP-Sun1-TM-C acts in a dominant-negative manner on endogenous Sun1 and Nesprin-2. COS7 cells expressing GFP-Sun1-TM-C were stained using specific antibodies to Sun1 (B,C) and Nesprin-2 (E,F). (A-F) Confocal images illustrating that GFP-Sun1-TM-C (transfected cells are indicated by arrows) interferes with the localisation of endogenous Sun1 (B and C, arrows) and Nesprin-2 (E and F, arrows). Note the differences in the Sun1 and Nesprin-2 staining pattern in transfected (arrows) versus untransfected (arrowheads) cells. (G) Histogram illustrating the displacement effects of the GFP-Sun1-TM-C fusion on the endogenous Sun1 and Nesprin-2 proteins. Data were obtained by evaluating 200 transfected cells. Bars, 7 µm.





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