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Fig. 8. Lamin A/C does not influence the NE localisation of Sun1. (A-C) Wild type (A) and lamin A/C knockout (B and C) mouse dermal fibroblasts were transfected with the mouse GFP-Sun1 and GFP-Sun1-N+2TM fusion proteins. Transiently transfected cells were processed for direct fluorescence microscopy. Note that both GFP fusion proteins localise to the nuclear envelope in the absence of lamin A/C (B and C). (D-F) Lamin A/C-/- fibroblasts were transfected with plasmid encoding V5-tagged human Sun1 and processed for immunofluorescence using antibodies to V5 and the LAP2{alpha}. (G-I) HeLa cells stably expressing human Sun1 (V5-tagged) were transiently transfected with a plasmid coding for the dominant negative GFP-lamin B1{Delta}2+ protein and stained for the V5 epitope. Images were obtained by confocal microscopy. Bars, 6 µm.





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