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Fig. 1. Subcellular distribution of rp3. (A) Confocal images of HeLa cells double labeled for rp3 and IC. rp3 appears at the centrosome (open arrow, left) and microtubule plus-end, which often colocalized with IC in the cytoplasm (arrows, middle). The focal plane was set so that both cytoplasmic and nuclear signals of rp3 are shown in a single image. In the nucleus, rp3 appears on punctate structures that were occasionally associated with IC labeling (arrowheads, right). Nuclei are circled. (B) Proteins from cytosolic and nuclear fractions of HeLa, HEK and dissected brain regions were analyzed by immunoblotting with the indicated antibodies. (C) Immunolabeling of endogenous rp3 and PML in paraformaldehyde-fixed and Triton X-100-extracted HeLa cells (top panels) or in Triton X-100-extracted, paraformaldehyde-fixed and DNaseI-digested HeLa cells (bottom panels). Lack of DAPI signal in the bottom row indicates complete DNA removal. Bar, 5 µm.





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