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Fig. 4. Nuclear transport of SATB1 and rp3. (A) HEK cells triple transfected with pTet-off, TRE-Flag-SATB1 and myc-p50 were cultured in the presence of doxycycline for 24 hours and switched to regular medium for an additional 24 hours prior to fixation. The fixed cells were double-labeled with anti-Flag and anti-myc (top) or anti- ${gamma}$ -adaptin and anti-myc antibodies (bottom). The centrosomal Golgi distributions in nontransfected cells are indicated (arrowheads). Dotted outlines represent cell margins. (B) Immunoblotting of total cell lysate extracts obtained from cells transfected with either rp3 siRNA or control siRNA and probed with anti-ß-tubulin and anti-rp3. (C) HEK cells were triple transfected with pTet-off, TRE-Flag-SATB1, together with either rp3 siRNA or control siRNA. The Flag-SATB1 expression was manipulated as described in A and the cells were immunolabeled with anti-Flag mAb. Bar, 5 µm.

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