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Fig. 5. SATB1 association with rp3 free from dynein complex and IC. (A) Cytosolic and nuclear extracts of HEK cells sedimented in a 5-20% linear sucrose gradient. Fractions were collected, analyzed by SDS-PAGE and immunoblotted with the indicated antibodies. Each nuclear fraction was immunoprecipitated (IP) with anti-rp3 or control rabbit IgG and immunoblotted (IB) with anti-SATB1 antibody (bottom panel). (B) HEK nuclear extracts were immunoprecipitated with either anti-IC or anti-rp3 and immunoblotted with the indicated antibodies. Immunoblots of total inputs are also shown. (C) Nuclear extracts underwent sequential immunoprecipitation procedures. The immunoprecipitates were subjected to immunoblotting with the indicated antibodies. Location of standards for 3.5S, 11.3S and 19S are indicated.





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