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Fig. 6. rp3 assembly within the SATB-1-Bcl2 MAR DNA complexes. Radiolabeled Bcl2 MAR was incubated with nuclear extracts of HEK cells transfected with SATB1 (lanes 1-5) or SATB1 together with Flag-rp3 (lanes 6-14). The indicated antibodies were added into each reaction. IC-depleted nuclear extracts were used in lanes 11-14. Arrows indicate the SATB1-containing Bcl2 MAR complexes (I/II). Asterisks label the slower migrating complexes generated by anti-SATB1 (lanes 2, 7, 12) and anti-Flag antibodies (lanes 8, 13).