spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. rp3 involvement in SATB1-mediated transcriptional repression activity and gene regulation of Bcl2. (A) HEK cells were transfected with pGL2-5Gal, pTK-RL plus the indicated plasmids. The luciferase activity was measured and that of pcDNA3.1-transfected cells was taken to be 100% (column 5). (B) Luciferase assays of HEK cells transfected with pTK-RL, pGL2-5Gal, pGal4DB-SATB1 and rp3 siRNA or control siRNA. The luciferase activity obtained from cells transfected with pcDNA3.1 and treated with control siRNA was taken to be 100%. (C) rp3 reversed SATB1 suppression effect on Bcl2-MAR. Luciferase assays were performed using lysates of HEK cells transfected with pTK-RL, Bcl2-MAR-Luc and plasmid combination as indicated. The activity of cells transfected with pRK5 was taken as 100%. (D) Bcl2 mRNA levels were measured by real-time RT-PCR using RNAs isolated 24 hours after transfection with the indicated plasmids. The Bcl2 mRNA level of pRK5-transfected cells was taken to be 100%. Significant differences in luciferase activity and mRNA levels were observed (**P<0.01) in indicated cells compared to levels in the control. All values represent the mean±s.e.m. of three separate experiments.





Right arrow Return to article