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Fig. 5. Dynamics of cell-substrate contact areas during shear-flow-induced motility. Ax2 cells undergoing shear-flow-induced motility (
=2.4 Pa) in the presence of the indicated calcium concentration were imaged at high resolution under RICM and morphology of cell-substrate contact areas was analyzed as described in Materials and Methods. (A) Growth rates of gained (left) and lost (right) contact areas are represented as a function of time, for individual cells recorded at the indicated calcium concentration. Average peak height and frequency are determined from such recordings. (B,C) Average peak height and frequency are plotted as a function of cell speed, for ten individual cells. 
, protrusions; 
, retractions. In B, the dotted line is a linear least square fit of all data points (slope 7.9 µm; ordinate at origin 1.7 µm2 second-1). Standard deviations for protrusion and retraction peak heights are 1.9 and 1.3 µm2 second-1, respectively. In C, the dotted lines represent the mean values of the retraction and protrusion frequencies.
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