spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Time-course of HypF-N binding to HeLa and Hend cells (either normal or enriched in membrane cholesterol). Both cell lines were exposed for 5, 10, 15, 20, 30, 60 minutes to 2.0 µM HypF-N pre-fibrillar aggregates and then washed twice with PBS. The residual aggregate-cell complex was stained with 2.0 µM Congo Red for 20 minutes. Under these conditions, Congo Red-staining is a measure of the amount of prefibrillar aggregates adsorbed to cell membrane surface. Binding of HypF-N aggregates to Hend cell membranes was highly reduced following supplementation of the culture media with 0.5 mM water-soluble cholesterol.





Right arrow Return to article