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Fig. 4. Time course of ROS accumulation. Confocal microscopy analysis of the redox status of two different cell lines exposed for 15, 30, 60, 180 minutes to 2.0 µM HypF-N pre-fibrillar aggregates. ROS production was checked by incubating the exposed cells, for 10 minutes, in the presence of the redox fluorescent probe CM-H2 DCFA. For technical details see under Materials and Methods. The values shown are means of three experiments carried out in duplicate.





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