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Fig. 6. Continuous confocal microscopy analysis of intracellular free Ca2+ levels in unfixed Hend and HeLa cells. Intracellular free Ca2+ levels were imaged by confocal microscopy using the fluorescent dye Fluo-3AM as a probe as described under Materials and Methods. Cells were exposed for 20 minutes to 2.0 µM HypF-N aggregates. Fluorescence is expressed as fractional change above the resting baseline, {Delta}F/F, where F is the average baseline fluorescence before aggregate exposure and {Delta}F is the fluorescence change over the baseline. The values shown are means ±s.d. of three independent experiments each carried out in triplicate. Bar, 30 µm.





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